Chromatography is a powerful analytical technique used to separate and analyze complex mixtures. This post will provide an overview of some common chromatography methods, their principles, steps, uses and examples. What is Chromatography? Chromatography is a laboratory technique in which a mixture is separated into its individual components. It relies on the differential distribution of the sample components between a moving fluid mobile phase and a stationary phase to achieve separation. Chromatography Definition: Chromatography is a laboratory technique for the separation of a mixture into its constituent parts. Stationary Phase : The stationary phase is the immobile phase fixed in place in a chromatography column. It can be a solid or liquid. Mobile Phase: The mobile phase is the solvent that moves through the chromatography column carrying the sample. 1. Affinity Chromatography - Principle: Based on specific biological interactions between antibody and antigen, enzyme and substra